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小鼠6酮前列腺素F1a酶免試劑盒,(6-keto-PGF1a)ELISA檢測(cè)試劑盒 ,英文名:6-keto-PGF1a ELISA Kit ,英文縮寫:6-keto-PGF1a ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠6酮前列腺素F1aELISA檢測(cè)試劑盒,6-keto-PGF1a 檢測(cè)試劑盒, 6-keto-PGF1a 檢測(cè)試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠6酮前列腺素F1a酶免試劑盒,(6-keto-PGF1a)ELISA檢測(cè)試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測(cè)定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測(cè)中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競(jìng)爭(zhēng)法及定量檢測(cè)影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無(wú)關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測(cè)讀儀(酶標(biāo)儀),比色法判讀可選擇單波長(zhǎng)或雙波長(zhǎng),根據(jù)反應(yīng)液顏色的不同選用不同波長(zhǎng)的濾光片,以空白孔校零測(cè)定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
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成纖維細(xì)胞生長(zhǎng)因子6(FGF6)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 6 (FGF6)96TY17563Homo sapiens (Human)
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成纖維細(xì)胞生長(zhǎng)因子6(FGF6)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 6 (FGF6)96TY17565Rattus norvegicus (Rat)
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FMS樣酪氨酸激酶3配體(Flt3L)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for FMS Like Tyrosine Kinase 3 Ligand (Flt3L)96TY17571Homo sapiens (Human)
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中性粒細(xì)胞激活蛋白3(NAP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Neutrophil Activating Protein 3 (NAP3)96TY17573Homo sapiens (Human)
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白介素3調(diào)節(jié)核因子(NFIL3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Nuclear Factor, Interleukin 3 Regulated (NFIL3)96TY17576Homo sapiens (Human)
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PDGFA關(guān)聯(lián)蛋白1(PDAP1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for PDGFA Associated Protein 1 (PDAP1)96TY17579Homo sapiens (Human)
PDGFA關(guān)聯(lián)蛋白1(PDAP1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for PDGFA Associated Protein 1 (PDAP1)96TY17580Mus musculus (Mouse)
PDGFA關(guān)聯(lián)蛋白1(PDAP1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for PDGFA Associated Protein 1 (PDAP1)96TY17581Rattus norvegicus (Rat)
小斑型POZ蛋白(SPOP)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Speckle Type POZ Protein (SPOP)96TY17582Homo sapiens (Human)
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小斑型POZ蛋白(SPOP)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Speckle Type POZ Protein (SPOP)96TY17584Rattus norvegicus (Rat)
正輔因子4(PC4)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Positive Cofactor 4 (PC4)96TY17585Homo sapiens (Human)
正輔因子4(PC4)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Positive Cofactor 4 (PC4)96TY17586Mus musculus (Mouse)
正輔因子4(PC4)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Positive Cofactor 4 (PC4)96TY17587Rattus norvegicus (Rat)
胰島素生長(zhǎng)因子樣家族成員2(IGFL2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 2 (IGFL2)96TY17588Homo sapiens (Human)
胰島素生長(zhǎng)因子樣家族成員3(IGFL3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 3 (IGFL3)96TY17589Homo sapiens (Human)
胰島素生長(zhǎng)因子樣家族成員3(IGFL3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 3 (IGFL3)96TY17590Mus musculus (Mouse)
胰島素生長(zhǎng)因子樣家族成員3(IGFL3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 3 (IGFL3)96TY17591Rattus norvegicus (Rat)
胰島素生長(zhǎng)因子樣家族成員4(IGFL4)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 4 (IGFL4)96TY17592Homo sapiens (Human)
胰島素生長(zhǎng)因子樣家族成員1(IGFL1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 1 (IGFL1)96TY17593Homo sapiens (Human)
趨化素樣因子超家族成員8(CKLFSF8)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Chemokine Like Factor Superfamily 8 (CKLFSF8)96TY17594Homo sapiens (Human)
趨化素樣因子超家族成員8(CKLFSF8)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Chemokine Like Factor Superfamily 8 (CKLFSF8)96TY17595Mus musculus (Mouse)
各種類型ELISA測(cè)定時(shí),一般需經(jīng)過以下步驟:固相包被→加待測(cè)樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。