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小鼠血管緊張素Ⅱ酶免試劑盒,(ANG-Ⅱ)ELISA檢測試劑盒,英文名:ANG-Ⅱ ELISA Kit,英文縮寫:ANG-Ⅱ ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠血管緊張素ⅡELISA檢測試劑盒,ANG-Ⅱ 檢測試劑盒,ANG-Ⅱ 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠血管緊張素Ⅱ酶免試劑盒,(ANG-Ⅱ)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
促腎上腺皮質(zhì)素釋放激素受體2(CRHR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Corticotropin Releasing Hormone Receptor 2 (CRHR2)48T/96TY13585Rattus norvegicus (Rat)
乙酰輔酶A乙酰轉(zhuǎn)移酶2(ACAT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acetyl Coenzyme A Acetyltransferase 2 (ACAT2)48T/96TY13586Homo sapiens (Human)
線粒體甘油-3-磷酸酰基轉(zhuǎn)移酶(GPAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM)48T/96TY13587Homo sapiens (Human)
炭疽熱毒素受體2(ANTXR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anthrax Toxin Receptor 2 (ANTXR2)48T/96TY13588Homo sapiens (Human)
絲裂原激活蛋白激酶激酶激酶6(MAP3K6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase Kinase Kinase 6 (MAP3K6)48T/96TY13589Rattus norvegicus (Rat)
血管生成素樣蛋白4(ANGPTL4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin Like Protein 4 (ANGPTL4)48T/96TY13590Rattus norvegicus (Rat)
血管生成素樣蛋白3(ANGPTL3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin Like Protein 3 (ANGPTL3)48T/96TY13591Rattus norvegicus (Rat)
再生蛋白1(NEO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neogenin 1 (NEO1)48T/96TY13592Homo sapiens (Human)
鐵蛋白(FE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ferritin (FE)48T/96TY13593Rattus norvegicus (Rat)
過氧化物酶體增殖物激活受體γ(PPARγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma (PPARg)48T/96TY13594Bos taurus; Bovine (Cattle)
鈉/鉀離子轉(zhuǎn)運ATP酶β3肽(ATP1β3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3)48T/96TY13595Mus musculus (Mouse)
兒茶酚-O-甲基轉(zhuǎn)移酶(COMT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Catechol-O-Methyltransferase (COMT)48T/96TY13596Mus musculus (Mouse)
神經(jīng)突蛋白1(NRN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuritin 1 (NRN1)48T/96TY13597Rattus norvegicus (Rat)
血小板激活因子(PAF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Activating Factor (PAF)48T/96TY13598Rhesus monkey (Simian)
Pim-1原癌基因(PIM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pim-1 Oncogene (PIM1)48T/96TY13599Homo sapiens (Human)
Pim-3原癌基因(PIM3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pim-3 Oncogene (PIM3)48T/96TY13600Homo sapiens (Human)
Pim-2原癌基因(PIM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pim-2 Oncogene (PIM2)48T/96TY13601Homo sapiens (Human)
羧酸酯酶1(CES1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carboxylesterase 1 (CES1)48T/96TY13602Rattus norvegicus (Rat)
嘌呤能受體P2X7(P2RX7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7)48T/96TY13603Homo sapiens (Human)
酪氨酸3/色氨酸5單加氧酶激活蛋白ζ(YWHAζ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz)48T/96TY13604Homo sapiens (Human)
Ⅻ型膠原(COL12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type XII (COL12)48T/96TY13605Mus musculus (Mouse)
磷脂酰肌醇特異性磷酯酶Cγ2(PLCγ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase C Gamma 2, Phosphatidylinositol Specific (PLCg2)48T/96TY13606Homo sapiens (Human)
Ki-67蛋白(Ki67P)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ki-67 Protein (Ki67P)48T/96TY13607Mus musculus (Mouse)
雙特異性磷酸酶5(DUSP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dual Specificity Phosphatase 5 (DUSP5)48T/96TY13608Homo sapiens (Human)
鈣結(jié)合蛋白(CALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calbindin (CALB)48T/96TY13609Homo sapiens (Human)
雙糖鏈蛋白聚糖(BGN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Biglycan (BGN)48T/96TY13610Mus musculus (Mouse)
神經(jīng)源性絲氨酸蛋白酶抑制劑(NSP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuroserpin (NSP)48T/96TY13611Homo sapiens (Human)
70kDa熱休克蛋白結(jié)合蛋白1(HSPBP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 70kDa Binding Protein 1 (HSPBP1)48T/96TY13612Homo sapiens (Human)
多型性腺瘤基因樣蛋白1(PLAGL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pleiomorphic Adenoma Gene Like Protein 1 (PLAGL1)48T/96TY13613Homo sapiens (Human)
雙特異性磷酸酶1(DUSP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dual Specificity Phosphatase 1 (DUSP1)48T/96TY13614Homo sapiens (Human)
網(wǎng)鈣蛋白2(RCN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Reticulocalbin 2 (RCN2)48T/96TY13615Mus musculus (Mouse)
電子傳遞黃素蛋白脫氫酶(ETFDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Electron Transferring Flavoprotein Dehydrogenase (ETFDH)48T/96TY13616Homo sapiens (Human)
低密度脂蛋白受體相關(guān)蛋白1(LRP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Low Density Lipoprotein Receptor Related Protein 1 (LRP1)48T/96TY13617Mus musculus (Mouse)
細(xì)胞色素P450家族成員26A1(CYP26A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome P450 26A1 (CYP26A1)48T/96TY13618Homo sapiens (Human)
絲氨酸肽酶抑制因子Kazal型5(SPINK5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Serine Peptidase Inhibitor Kazal Type 5 (SPINK5)48T/96TY13619Homo sapiens (Human)
細(xì)胞色素P450家族成員3A4(CYP3A4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome P450 3A4 (CYP3A4)48T/96TY13620Mus musculus (Mouse)
天冬氨酸轉(zhuǎn)氨酶2(AST2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aspartate Aminotransferase 2 (AST2)48T/96TY13621Mus musculus (Mouse)
核氧化還原蛋白(NXN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleoredoxin (NXN)48T/96TY13622Homo sapiens (Human)
視網(wǎng)膜劈裂蛋白(RS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Retinoschisin (RS)48T/96TY13623Homo sapiens (Human)
膽固醇-25-羥化酶(CH25H)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholesterol-25-Hydroxylase (CH25H)48T/96TY13624Homo sapiens (Human)
胱硫醚β合酶(CβS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cystathionine Beta Synthase (CbS)48T/96TY13625Mus musculus (Mouse)
再生胰島衍生蛋白3α(REG3α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 3 Alpha (REG3a)48T/96TY13626Rattus norvegicus (Rat)
MARCKS相關(guān)蛋白(MARCKSL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for MARCKS Related Protein (MARCKSL1)48T/96TY13627Homo sapiens (Human)
L1-細(xì)胞粘附分子(L1CAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for L1-Cell Adhesion Molecule (L1CAM)48T/96TY13628Homo sapiens (Human)
唾液酸結(jié)合Ig樣凝集素10(SIGLEC10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sialic Acid Binding Ig Like Lectin 10 (SIGLEC10)48T/96TY13629Homo sapiens (Human)
二肽基肽酶9(DPP9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dipeptidyl Peptidase 9 (DPP9)48T/96TY13630Mus musculus (Mouse)
二肽基肽酶8(DPP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dipeptidyl Peptidase 8 (DPP8)48T/96TY13631Mus musculus (Mouse)
囊泡乙酰膽堿轉(zhuǎn)運蛋白(VAChT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vesicular Acetylcholine Transporter (VAChT)48T/96TY13632Homo sapiens (Human)
核因子κB(NFκB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Factor Kappa B (NFkB)48T/96TY13633Mus musculus (Mouse)
分泌型卷曲相關(guān)蛋白5(SFRP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secreted Frizzled Related Protein 5 (SFRP5)48T/96TY13634Rattus norvegicus (Rat)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。