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小鼠Ⅰ型膠原C端肽酶免試劑盒,(CTX-Ⅰ)ELISA檢測試劑盒 ,英文名:CTX-Ⅰ ELISA Kit ,英文縮寫:CTX-Ⅰ ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠Ⅰ型膠原C端肽ELISA檢測試劑盒,CTX-Ⅰ 檢測試劑盒, CTX-Ⅰ 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠Ⅰ型膠原C端肽酶免試劑盒,(CTX-Ⅰ)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
干擾素調(diào)節(jié)因子9(IRF9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Regulatory Factor 9 (IRF9)48T/96TY12585Homo sapiens (Human)
分泌型卷曲相關(guān)蛋白1(SFRP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secreted Frizzled Related Protein 1 (SFRP1)48T/96TY12586Mus musculus (Mouse)
整合素β1(ITGβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Integrin Beta 1 (ITGb1)48T/96TY12587Homo sapiens (Human)
脂肪酸轉(zhuǎn)運(yùn)蛋白5(FATP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fatty Acid Transport Protein 5 (FATP5)48T/96TY12588Homo sapiens (Human)
血管生成素樣蛋白3(ANGPTL3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin Like Protein 3 (ANGPTL3)48T/96TY12589Mus musculus (Mouse)
亮氨酸豐富重復(fù)LGI家族成員3(LGI3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leucine Rich Repeat LGI Family, Member 3 (LGI3)48T/96TY12590Homo sapiens (Human)
多聚蛋白1(MMRN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Multimerin 1 (MMRN1)48T/96TY12591Homo sapiens (Human)
低氧誘導(dǎo)因子2α(HIF2α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hypoxia Inducible Factor 2 Alpha (HIF2a)48T/96TY12592Homo sapiens (Human)
低氧誘導(dǎo)因子2α(HIF2α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hypoxia Inducible Factor 2 Alpha (HIF2a)48T/96TY12593Mus musculus (Mouse)
醛脫氫酶1家族成員A2(ALDH1A2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2)48T/96TY12594Homo sapiens (Human)
內(nèi)酰胺酶β(LACTβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactamase Beta (LACTb)48T/96TY12595Homo sapiens (Human)
無翅型MMTV整合位點家族成員5A(WNT5A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)48T/96TY12596Homo sapiens (Human)
解整合素金屬蛋白酶12(ADAM12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 12 (ADAM12)48T/96TY12597Homo sapiens (Human)
趨化因子C-X3-C-基元受體1(CX3CR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-X3-C-Motif Receptor 1 (CX3CR1)48T/96TY12598Mus musculus (Mouse)
107kDa肌醇多聚磷酸-4-磷酸酶Ⅰ型(INPP4A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A)48T/96TY12599Homo sapiens (Human)
107kDa肌醇多聚磷酸-4-磷酸酶Ⅰ型(INPP4A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A)48T/96TY12600Mus musculus (Mouse)
層粘連蛋白β3(LAMβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Laminin Beta 3 (LAMb3)48T/96TY12601Homo sapiens (Human)
融合蛋白(FUS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fusion (FUS)48T/96TY12602Mus musculus (Mouse)
黑素轉(zhuǎn)鐵蛋白(MFI2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Melanotransferrin (MFI2)48T/96TY12603Homo sapiens (Human)
視神經(jīng)病變誘導(dǎo)反應(yīng)蛋白(OPTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Optineurin (OPTN)48T/96TY12604Mus musculus (Mouse)
8-羥基鳥嘌呤糖苷酶1(OGG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Oxoguanine Glycosylase 1 (OGG1)48T/96TY12605Homo sapiens (Human)
尿嘧啶DNA糖基化酶(UNG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Uracil DNA Glycosylase (UNG)48T/96TY12606Homo sapiens (Human)
胸苷嘧啶DNA糖基化酶(TDG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thymine DNA Glycosylase (TDG)48T/96TY12607Homo sapiens (Human)
單鏈選擇性單功能尿嘧啶DNA糖基化酶1(SMUG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1)48T/96TY12608Homo sapiens (Human)
N-甲基嘌呤DNA糖基化酶(MPG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for N-Methylpurine DNA Glycosylase (MPG)48T/96TY12609Homo sapiens (Human)
MutY同源物(MUTYH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for MutY Homolog (MUTYH)48T/96TY12610Homo sapiens (Human)
激活蛋白C(APC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activated Protein C (APC)48T/96TY12611Sus scrofa; Porcine (Pig)
激活蛋白C(APC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activated Protein C (APC)48T/96TY12612Rattus norvegicus (Rat)
嗜鉻蛋白B(CHGB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chromogranin B (CHGB)48T/96TY12613Homo sapiens (Human)
腎酶(RNLS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Renalase (RNLS)48T/96TY12614Homo sapiens (Human)
原鈣黏素15(PCDH15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protocadherin 15 (PCDH15)48T/96TY12615Mus musculus (Mouse)
RAD51同源物(RAD51)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for RAD51 Homolog (RAD51)48T/96TY12616Homo sapiens (Human)
黑素瘤抑制性活性蛋白1(MIA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Melanoma Inhibitory Activity Protein 1 (MIA1)48T/96TY12617Homo sapiens (Human)
極低密度脂蛋白(VLDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Very Low Density Lipoprotein (VLDL)48T/96TY12618Mus musculus (Mouse)
兒茶酚-O-甲基轉(zhuǎn)移酶(COMT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Catechol-O-Methyltransferase (COMT)48T/96TY12619Homo sapiens (Human)
苯酚磺基轉(zhuǎn)移酶(PST)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phenol Sulfotransferase (PST)48T/96TY12620Homo sapiens (Human)
孕烷X受體(PXR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pregnane X Receptor (PXR)48T/96TY12621Homo sapiens (Human)
骨骼肌特異性受體酪氨酸激酶(MUSK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK)48T/96TY12622Homo sapiens (Human)
糖化血紅蛋白(HbA1C)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycated Hemoglobin (HbA1C)48T/96TY12623Homo sapiens (Human)
抑肽酶(AP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aprotinin (AP)48T/96TY12624Bos taurus; Bovine (Cattle)
皮質(zhì)醇結(jié)合球蛋白(CBG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Corticosteroid Binding Globulin (CBG)48T/96TY12625Rattus norvegicus (Rat)
FK506結(jié)合蛋白5(FKBP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for FK506 Binding Protein 5 (FKBP5)48T/96TY12626Homo sapiens (Human)
單酰甘油-O-?;D(zhuǎn)移酶2(MOGAT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monoacylglycerol-O-acyltransferase 2 (MOGAT2)48T/96TY12627Homo sapiens (Human)
二酰甘油-O-?;D(zhuǎn)移酶同源物2(DGAT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2)48T/96TY12628Homo sapiens (Human)
二酰甘油-O-?;D(zhuǎn)移酶同源物1(DGAT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1)48T/96TY12629Homo sapiens (Human)
白細(xì)胞衍生趨化因子1(LECT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukocyte Cell Derived Chemotaxin 1 (LECT1)48T/96TY12630Mus musculus (Mouse)
足盂蛋白(PCX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Podocalyxin (PCX)48T/96TY12631Mus musculus (Mouse)
夏科雷登氏結(jié)晶蛋白(CLC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Charcot Leyden Crystal Protein (CLC)48T/96TY12632Mus musculus (Mouse)
ⅫB組磷脂酶A2(PLA2G12B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2, Group XIIB (PLA2G12B)48T/96TY12633Homo sapiens (Human)
DNA甲基轉(zhuǎn)移酶3B(DNMT3B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for DNA Methyltransferase 3B (DNMT3B)48T/96TY12634Mus musculus (Mouse)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。