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小鼠抗單鏈DNA抗體/變性DNA抗體酶免試劑盒,(ssDNA)ELISA檢測試劑盒 ,英文名:ssDNA ELISA Kit ,英文縮寫:ssDNA ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠抗單鏈DNA抗體/變性DNA抗體ELISA檢測試劑盒,ssDNA 檢測試劑盒, ssDNA 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠抗單鏈DNA抗體/變性DNA抗體酶免試劑盒,(ssDNA)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
脂質(zhì)運(yùn)載蛋白1(LCN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipocalin 1 (LCN1)48T/96TY12185Rattus norvegicus (Rat)
ⅩⅦ型膠原(COL17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type XVII (COL17)48T/96TY12186Homo sapiens (Human)
胸腺素β10(TMSβ10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thymosin Beta 10 (TMSb10)48T/96TY12187Homo sapiens (Human)
胞漿抗蛋白水解酶3(CAP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytoplasmic Antiproteinase 3 (CAP3)48T/96TY12188Homo sapiens (Human)
肝配蛋白A1(EFNA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ephrin A1 (EFNA1)48T/96TY12189Homo sapiens (Human)
谷胱甘肽S轉(zhuǎn)移酶θ1(GSTθ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Theta 1 (GSTt1)48T/96TY12190Homo sapiens (Human)
谷胱甘肽S轉(zhuǎn)移酶θ1(GSTθ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Theta 1 (GSTt1)48T/96TY12191Mus musculus (Mouse)
谷胱甘肽S轉(zhuǎn)移酶θ1(GSTθ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Theta 1 (GSTt1)48T/96TY12192Rattus norvegicus (Rat)
脂多糖(LPS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipopolysaccharides (LPS)48T/96TY12193General
胰島素樣生長因子結(jié)合蛋白5(IGFBP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 5 (IGFBP5)48T/96TY12194Homo sapiens (Human)
趨化因子C-C-基元配體14(CCL14)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-C-Motif Ligand 14 (CCL14)48T/96TY12195Homo sapiens (Human)
皮敵菌素(DCD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dermcidin (DCD)48T/96TY12196Rattus norvegicus (Rat)
丙氨酸氨肽酶(AAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alanine Aminopeptidase (AAP)48T/96TY12197Homo sapiens (Human)
催乳素(PRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prolactin (PRL)48T/96TY12198Bos taurus; Bovine (Cattle)
Ⅰ型膠原α1(COL1α1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type I Alpha 1 (COL1a1)48T/96TY12199Rattus norvegicus (Rat)
解整合素金屬蛋白酶17(ADAM17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 17 (ADAM17)48T/96TY12200Homo sapiens (Human)
S100鈣結(jié)合蛋白A7(S100A7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A7 (S100A7)48T/96TY12201Homo sapiens (Human)
免疫球蛋白結(jié)合蛋白1(IGBP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin Binding Protein 1 (IGBP1)48T/96TY12202Bos taurus; Bovine (Cattle)
黃體激素(LH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Luteinizing Hormone (LH)48T/96TY12203Capra hircus; Caprine (Goat)
微管關(guān)聯(lián)蛋白τ(MAPτ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Microtubule Associated Protein Tau (MAPt)48T/96TY12204Mus musculus (Mouse)
微管關(guān)聯(lián)蛋白τ(MAPτ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Microtubule Associated Protein Tau (MAPt)48T/96TY12205Rattus norvegicus (Rat)
血幼素(HJV)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hemojuvelin (HJV)48T/96TY12206Mus musculus (Mouse)
Ⅱ型膠原α1(COL2α1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type II Alpha 1 (COL2a1)48T/96TY12207Homo sapiens (Human)
乙醇脫氫酶1(ADH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alcohol Dehydrogenase 1 (ADH1)48T/96TY12208Mus musculus (Mouse)
線粒體乙醛脫氫酶(ALDM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aldehyde Dehydrogenase, Mitochondrial (ALDM)48T/96TY12209Mus musculus (Mouse)
乙醇脫氫酶7(ADH7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alcohol Dehydrogenase 7 (ADH7)48T/96TY12210Mus musculus (Mouse)
磷酸甘油酸酯激酶1(PGK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphoglycerate Kinase 1 (PGK1)48T/96TY12211Homo sapiens (Human)
過氧化氫酶(CAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Catalase (CAT)48T/96TY12212Homo sapiens (Human)
肝型脂肪酸結(jié)合蛋白(FABP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fatty Acid Binding Protein 1, Liver (FABP1)48T/96TY12213Sus scrofa; Porcine (Pig)
孕酮(Pg)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Progesterone (Pg)48T/96TY12214General
微管關(guān)聯(lián)蛋白2(MAP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Microtubule Associated Protein 2 (MAP2)48T/96TY12215Mus musculus (Mouse)
血管內(nèi)皮生長因子受體1(VEGFR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor Receptor 1 (VEGFR1)48T/96TY12216Sus scrofa; Porcine (Pig)
晚期糖基化終末產(chǎn)物受體(AGER)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Advanced Glycosylation End Product Specific Receptor (AGER)48T/96TY12217Mus musculus (Mouse)
載脂蛋白M(APOM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein M (APOM)48T/96TY12218Homo sapiens (Human)
胰島素樣生長因子結(jié)合蛋白7(IGFBP7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 7 (IGFBP7)48T/96TY12219Homo sapiens (Human)
α白蛋白(AFM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Afamin (AFM)48T/96TY12220Homo sapiens (Human)
免疫球蛋白M(IgM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin M (IgM)48T/96TY12221Chicken (Gallus)
脂肪酸合酶(FASN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fatty Acid Synthase (FASN)48T/96TY12222Homo sapiens (Human)
脂肪酸合酶(FASN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fatty Acid Synthase (FASN)48T/96TY12223Rattus norvegicus (Rat)
乳脂球表皮生長因子8(MFGE8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)48T/96TY12224Homo sapiens (Human)
野鼠色基因相關(guān)蛋白(AGRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Agouti Related Protein (AGRP)48T/96TY12225Rattus norvegicus (Rat)
激肽釋放酶1(KLK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 1 (KLK1)48T/96TY12226Rattus norvegicus (Rat)
胰腺再生蛋白Iα(REG1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 1 Alpha (REG1a)48T/96TY12227Rattus norvegicus (Rat)
胰腺特異轉(zhuǎn)錄因子1α(PTF1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pancreas Specific Transcription Factor 1a (PTF1a)48T/96TY12228Homo sapiens (Human)
含血管性血友病因子A域蛋白3A(vWA3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Von Willebrand Factor A Domain Containing Protein 3A (vWA3A)48T/96TY12229Mus musculus (Mouse)
補(bǔ)體因子P(CFP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Factor P (CFP)48T/96TY12230Homo sapiens (Human)
腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體(TRAIL)檢測試劑盒(酶聯(lián)免疫吸附試驗法),(ssDNA)ELISA檢測試劑盒ELISA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)48T/96TY12231Homo sapiens (Human)
腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體(TRAIL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)48T/96TY12232Mus musculus (Mouse)
核心蛋白聚糖(DCN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Decorin (DCN)48T/96TY12233Homo sapiens (Human)
磷脂轉(zhuǎn)運(yùn)蛋白(PLTP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipid Transfer Protein (PLTP)48T/96TY12234Homo sapiens (Human)
各種類型ELISA測定時,,(ssDNA)ELISA檢測試劑盒一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。