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小鼠β-防御素酶免試劑盒,(β-DF)ELISA檢測試劑盒 ,英文名:β-DF ELISA Kit ,英文縮寫:β-DF ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠β-防御素ELISA檢測試劑盒,β-DF 檢測試劑盒, β-DF 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠β-防御素酶免試劑盒,(β-DF)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
白介素15(IL15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 15 (IL15)48T/96TY11635Cavia (Guinea pig )
白介素15(IL15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 15 (IL15)48T/96TY11636Sus scrofa; Porcine (Pig)
Ⅳ型膠原α1(COL4α1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type IV Alpha 1 (COL4a1)48T/96TY11637Mus musculus (Mouse)
Ⅳ型膠原α1(COL4α1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type IV Alpha 1 (COL4a1)48T/96TY11638Rattus norvegicus (Rat)
血管內(nèi)皮生長因子B(VEGFB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor B (VEGFB)48T/96TY11639Bos taurus; Bovine (Cattle)
血管內(nèi)皮生長因子C(VEGFC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor C (VEGFC)48T/96TY11640Bos taurus; Bovine (Cattle)
血管內(nèi)皮生長因子D(VEGFD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor D (VEGFD)48T/96TY11641Bos taurus; Bovine (Cattle)
白介素15(IL15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 15 (IL15)48T/96TY11642Oryctolagus cuniculus (Rabbit)
血管生長素(ANG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiogenin (ANG)48T/96TY11643Sus scrofa; Porcine (Pig)
甲胎蛋白(αFP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-Fetoprotein (aFP)48T/96TY11644Bos taurus; Bovine (Cattle)
甲胎蛋白(αFP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-Fetoprotein (aFP)48T/96TY11645Canis familiaris; Canine (Dog)
甲胎蛋白(αFP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-Fetoprotein (aFP)48T/96TY11646Equus caballus; Equine (Horse)
脂聯(lián)素受體1(ADIPOR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adiponectin Receptor 1 (ADIPOR1)48T/96TY11647Homo sapiens (Human)
脂聯(lián)素受體1(ADIPOR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adiponectin Receptor 1 (ADIPOR1)48T/96TY11648Mus musculus (Mouse)
脂聯(lián)素受體1(ADIPOR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adiponectin Receptor 1 (ADIPOR1)48T/96TY11649Sus scrofa; Porcine (Pig)
脂聯(lián)素受體1(ADIPOR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adiponectin Receptor 1 (ADIPOR1)48T/96TY11650Rattus norvegicus (Rat)
脂聯(lián)素受體1(ADIPOR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adiponectin Receptor 1 (ADIPOR1)48T/96TY11651Rhesus monkey (Simian)
甲胎蛋白(αFP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-Fetoprotein (aFP)48T/96TY11652Chicken (Gallus)
甲胎蛋白(αFP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-Fetoprotein (aFP)48T/96TY11653Sus scrofa; Porcine (Pig)
甲胎蛋白(αFP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-Fetoprotein (aFP)48T/96TY11654Rhesus monkey (Simian)
激肽釋放酶3(KLK3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 3 (KLK3)48T/96TY11655Rhesus monkey (Simian)
血管生長素(ANG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiogenin (ANG)48T/96TY11656Equus caballus; Equine (Horse)
嗅素4(OLFM4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Olfactomedin 4 (OLFM4)48T/96TY11657Mus musculus (Mouse)
鱗狀細(xì)胞癌抗原2(SCCA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Squamous Cell Carcinoma Antigen 2 (SCCA2)48T/96TY11658Mus musculus (Mouse)
白介素16(IL16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 16 (IL16)48T/96TY11659Bos taurus; Bovine (Cattle)
白介素16(IL16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 16 (IL16)48T/96TY11660Sus scrofa; Porcine (Pig)
白介素17(IL17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 17 (IL17)48T/96TY11661Bos taurus; Bovine (Cattle)
白介素17(IL17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 17 (IL17)48T/96TY11662Equus caballus; Equine (Horse)
成纖維細(xì)胞生長因子受體1(FGFR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor Receptor 1 (FGFR1)48T/96TY11663Homo sapiens (Human)
轉(zhuǎn)化生長因子β2(TGFβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)48T/96TY11664Rhesus monkey (Simian)
成纖維細(xì)胞生長因子23(FGF23)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 23 (FGF23)48T/96TY11665Mus musculus (Mouse)
胰島素樣生長因子結(jié)合蛋白6(IGFBP6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY11666Rattus norvegicus (Rat)
胰島素樣生長因子結(jié)合蛋白5(IGFBP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 5 (IGFBP5)48T/96TY11667Rattus norvegicus (Rat)
轉(zhuǎn)化生長因子β2(TGFβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)48T/96TY11668Bos taurus; Bovine (Cattle)
轉(zhuǎn)化生長因子β2(TGFβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)48T/96TY11669Capra hircus; Caprine (Goat)
淋巴細(xì)胞胞漿蛋白1(LCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lymphocyte Cytosolic Protein 1 (LCP1)48T/96TY11670Homo sapiens (Human)
環(huán)胍氨酸肽(CCP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclical Citrullinated Peptide (CCP)48T/96TY11671Mus musculus (Mouse)
軟骨寡聚基質(zhì)蛋白(COMP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cartilage Oligomeric Matrix Protein (COMP)48T/96TY11672Mus musculus (Mouse)
線粒體乙醛脫氫酶(ALDM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aldehyde Dehydrogenase, Mitochondrial (ALDM)48T/96TY11673Rattus norvegicus (Rat)
腦型肌酸激酶(CKB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Creatine Kinase, Brain (CKB)48T/96TY11674Homo sapiens (Human)
胰島素樣生長因子1(IGF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 1 (IGF1)48T/96TY11675Cavia (Guinea pig )
維生素D4(VD4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vitamin D4 (VD4)48T/96TY11676General
脂聯(lián)素(ADP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adiponectin (ADP)48T/96TY11677Bos taurus; Bovine (Cattle)
內(nèi)脂素(VF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Visfatin (VF)48T/96TY11678Bos taurus; Bovine (Cattle)
血小板衍生生長因子AA(PDGFAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Derived Growth Factor AA (PDGFAA)48T/96TY11679Homo sapiens (Human)
主要堿性蛋白(MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Major Basic Protein (MBP)48T/96TY11680Sus scrofa; Porcine (Pig)
腎上腺髓質(zhì)素(ADM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adrenomedullin (ADM)48T/96TY11681Bos taurus; Bovine (Cattle)
腎上腺髓質(zhì)素(ADM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)小鼠β-防御素酶免試劑盒,(β-DF)ELISA檢測試劑盒ELISA Kit for Adrenomedullin (ADM)48T/96TY11682Sus scrofa; Porcine (Pig)
干擾素β(IFNβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Beta (IFNb)48T/96TY11683Bos taurus; Bovine (Cattle)
干擾素β(IFNβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Beta (IFNb)48T/96TY11684Canis familiaris; Canine (Dog)
各種類型ELISA測定時,小鼠β-防御素酶免試劑盒,(β-DF)ELISA檢測試劑盒一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。