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小鼠白介素15酶免試劑盒,(IL-15)ELISA檢測試劑盒,英文名:IL-15 ELISA Kit,英文縮寫:IL-15 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠白介素15ELISA檢測試劑盒,IL-15 檢測試劑盒,IL-15 檢測試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠白介素15酶免試劑盒,(IL-15)ELISA檢測試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競爭法及定量檢測影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)48T/96TY10885Rattus norvegicus (Rat)
組織金屬蛋白酶抑制因子1(TIMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)48T/96TY10886Homo sapiens (Human)
組織金屬蛋白酶抑制因子1(TIMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)48T/96TY10887Mus musculus (Mouse)
組織金屬蛋白酶抑制因子1(TIMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)48T/96TY10888Rattus norvegicus (Rat)
基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Matrix Metalloproteinase 9 (MMP9)48T/96TY10889Homo sapiens (Human)
基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Matrix Metalloproteinase 9 (MMP9)48T/96TY10890Mus musculus (Mouse)
基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Matrix Metalloproteinase 9 (MMP9)48T/96TY10891Rattus norvegicus (Rat)
尼克酰胺腺嘌呤二核苷酸磷酸氧化酶1()檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 ()48T/96TY10892Homo sapiens (Human)
尼克酰胺腺嘌呤二核苷酸磷酸氧化酶1()檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 ()48T/96TY10893Mus musculus (Mouse)
尼克酰胺腺嘌呤二核苷酸磷酸氧化酶1()檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 ()48T/96TY10894Rattus norvegicus (Rat)
尼克酰胺腺嘌呤二核苷酸磷酸氧化酶5(NOX5)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5 (NOX5)48T/96TY10895Homo sapiens (Human)
白介素16(IL16)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 16 (IL16)48T/96TY10896Chicken (Gallus)
cAMP應(yīng)答元件結(jié)合蛋白(CREB)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cyclic AMP Response Element Binding Protein (CREB)48T/96TY10897Rattus norvegicus (Rat)
端粒酶關(guān)聯(lián)蛋白1(TEP1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for omerase Associated Protein 1 (TEP1)48T/96TY10898Homo sapiens (Human)
端粒酶關(guān)聯(lián)蛋白1(TEP1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for omerase Associated Protein 1 (TEP1)48T/96TY10899Mus musculus (Mouse)
端粒酶關(guān)聯(lián)蛋白1(TEP1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for omerase Associated Protein 1 (TEP1)48T/96TY10900Rattus norvegicus (Rat)
腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2)48T/96TY10901Homo sapiens (Human)
腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2)48T/96TY10902Mus musculus (Mouse)
腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2)48T/96TY10903Rattus norvegicus (Rat)
表皮生長因子(EGF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Epidermal Growth Factor (EGF)48T/96TY10904Homo sapiens (Human)
表皮生長因子(EGF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Epidermal Growth Factor (EGF)48T/96TY10905Mus musculus (Mouse)
表皮生長因子(EGF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Epidermal Growth Factor (EGF)48T/96TY10906Rattus norvegicus (Rat)
白血病抑制因子受體(LIFR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Leukemia Inhibitory Factor Receptor (LIFR)48T/96TY10907Homo sapiens (Human)
白血病抑制因子受體(LIFR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Leukemia Inhibitory Factor Receptor (LIFR)48T/96TY10908Mus musculus (Mouse)
白血病抑制因子受體(LIFR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Leukemia Inhibitory Factor Receptor (LIFR)48T/96TY10909Rattus norvegicus (Rat)
白三烯B4(LTB4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Leukotriene B4 (LTB4)48T/96TY10910General
一氧化氮合酶運(yùn)輸因子(NOSTRIN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Nitric Oxide Synthase Trafficker (NOSTRIN)48T/96TY10911Homo sapiens (Human)
促性腺激素釋放激素(GnRH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Gonadotropin Releasing Hormone (GnRH)48T/96TY10912Rattus norvegicus (Rat)
促腎上皮質(zhì)激素釋放激素(CRH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Corticotropin Releasing Hormone (CRH)48T/96TY10913Rattus norvegicus (Rat)
促甲狀腺素釋放激素(TRH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Thyrotropin Releasing Hormone (TRH)48T/96TY10914Rattus norvegicus (Rat)
乙酰膽堿(ACH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Acetylcholine (ACH)48T/96TY10915General
肌紅蛋白(MYO)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Myoglobin (MYO)48T/96TY10916Rattus norvegicus (Rat)
肌紅蛋白(MYO)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Myoglobin (MYO)48T/96TY10917Mus musculus (Mouse)
白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 8 (IL8)48T/96TY10918Canis familiaris; Canine (Dog)
白介素1β(IL1β)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Beta (IL1b)48T/96TY10919Homo sapiens (Human)
白介素1β(IL1β)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Beta (IL1b)48T/96TY10920Mus musculus (Mouse)
白介素1β(IL1β)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Beta (IL1b)48T/96TY10921Rattus norvegicus (Rat)
白介素17(IL17)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 17 (IL17)48T/96TY10922Homo sapiens (Human)
白介素17(IL17)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 17 (IL17)48T/96TY10923Sus scrofa; Porcine (Pig)
白介素1δ(FIL1δ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Delta (FIL1d)48T/96TY10924Homo sapiens (Human)
白介素1δ(FIL1δ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Delta (FIL1d)48T/96TY10925Mus musculus (Mouse)
白介素1δ(FIL1δ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 1 Delta (FIL1d)48T/96TY10926Rattus norvegicus (Rat)
S100鈣結(jié)合蛋白B(S100B)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for S100 Calcium Binding Protein B (S100B)48T/96TY10927Homo sapiens (Human)
S100鈣結(jié)合蛋白B(S100B)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for S100 Calcium Binding Protein B (S100B)48T/96TY10928Rattus norvegicus (Rat)
S100鈣結(jié)合蛋白A11(S100A11)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for S100 Calcium Binding Protein A11 (S100A11)48T/96TY10929Homo sapiens (Human)
S100鈣結(jié)合蛋白A11(S100A11)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for S100 Calcium Binding Protein A11 (S100A11)48T/96TY10930Mus musculus (Mouse)
S100鈣結(jié)合蛋白A11(S100A11)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for S100 Calcium Binding Protein A11 (S100A11)48T/96TY10931Rattus norvegicus (Rat)
P選擇素(SELP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Selectin, Plaet (SELP)48T/96TY10932Homo sapiens (Human)
Ⅰ型膠原(COL1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Collagen Type I (COL1)48T/96TY10933Mus musculus (Mouse)
Ⅰ型膠原(COL1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Collagen Type I (COL1)48T/96TY10934Rattus norvegicus (Rat)
維生素B6(VB6)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vitamin B6 (VB6)48T/96TY10935General
Ⅱ型膠原(COL2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Collagen Type II (COL2)48T/96TY10936Homo sapiens (Human)
Ⅱ型膠原(COL2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Collagen Type II (COL2)48T/96TY10937Mus musculus (Mouse)
維生素C(VC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vitamin C (VC)48T/96TY10938General
Ⅱ型膠原(COL2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Collagen Type II (COL2)48T/96TY10939Rattus norvegicus (Rat)
Ⅲ型前膠原氨基端原肽(PⅢNP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Procollagen III N-Terminal Propeptide (PIIINP)48T/96TY10940Homo sapiens (Human)
Ⅲ型前膠原氨基端原肽(PⅢNP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Procollagen III N-Terminal Propeptide (PIIINP)48T/96TY10941Mus musculus (Mouse)
Ⅲ型前膠原氨基端原肽(PⅢNP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Procollagen III N-Terminal Propeptide (PIIINP)48T/96TY10942Rattus norvegicus (Rat)
25-羥基維生素D3(HVD3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for 25-Hydroxyvitamin D3 (HVD3)48T/96TY10943General
載脂蛋白B100(APOB100)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Apolipoprotein B100 (APOB100)48T/96TY10944Homo sapiens (Human)
載脂蛋白B100(APOB100)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Apolipoprotein B100 (APOB100)48T/96TY10945Rattus norvegicus (Rat)
載脂蛋白A2(APOA2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Apolipoprotein A2 (APOA2)48T/96TY10946Homo sapiens (Human)
載脂蛋白A2(APOA2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Apolipoprotein A2 (APOA2)48T/96TY10947Mus musculus (Mouse)
載脂蛋白A2(APOA2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Apolipoprotein A2 (APOA2)48T/96TY10948Rattus norvegicus (Rat)
P選擇素(SELP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Selectin, Plaet (SELP)48T/96TY10949Sus scrofa; Porcine (Pig)
層粘連蛋白α1(LAMα1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Laminin Alpha 1 (LAMa1)48T/96TY10950Homo sapiens (Human)
層粘連蛋白α1(LAMα1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Laminin Alpha 1 (LAMa1)48T/96TY10951Mus musculus (Mouse)
層粘連蛋白α1(LAMα1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Laminin Alpha 1 (LAMa1)48T/96TY10952Rattus norvegicus (Rat)
磷脂酶A2激活蛋白(PLAA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Phospholipase A2 Activating Protein (PLAA)48T/96TY10953Homo sapiens (Human)
堿性鞘磷脂磷酸二酯酶(Alk-Smase)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase)48T/96TY10954Rattus norvegicus (Rat)
各種類型ELISA測定時(shí),一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。