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小鼠胰島素樣生長因子2酶免試劑盒,(IGF-2)ELISA檢測試劑盒,英文名:IGF-2 ELISA Kit,英文縮寫:IGF-2 ,常見試劑盒規(guī)格:96T/48T,產品別名:小鼠胰島素樣生長因子2ELISA檢測試劑盒,IGF-2 檢測試劑盒,IGF-2 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠胰島素樣生長因子2酶免試劑盒,(IGF-2)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
P選擇素(SELP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Selectin, Plaet (SELP)48T/96TY10433Mus musculus (Mouse)
P選擇素(SELP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Selectin, Plaet (SELP)48T/96TY10434Rattus norvegicus (Rat)
Ⅰ型前膠原羧基端原肽(PⅠCP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Procollagen I C-Terminal Propeptide (PICP)48T/96TY10435Homo sapiens (Human)
Ⅰ型前膠原羧基端原肽(PⅠCP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Procollagen I C-Terminal Propeptide (PICP)48T/96TY10436Mus musculus (Mouse)
Ⅰ型前膠原羧基端原肽(PⅠCP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Procollagen I C-Terminal Propeptide (PICP)48T/96TY10437Rattus norvegicus (Rat)
Ⅰ型膠原(COL1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Collagen Type I (COL1)48T/96TY10438Homo sapiens (Human)
中性粒細胞明膠酶關聯脂質運載蛋白(NGAL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neutrophil Gelatinase Associated Lipocalin (NGAL)48T/96TY10439Sus scrofa; Porcine (Pig)
半胱氨酸蛋白酶抑制劑3(CST3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cystatin 3 (CST3)48T/96TY10440Sus scrofa; Porcine (Pig)
43kDa Tar DNA結合蛋白(TDP43)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tar DNA Binding Protein 43kDa (TDP43)48T/96TY10441Homo sapiens (Human)
結合珠蛋白(Hpt)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Haptoglobin (Hpt)48T/96TY10442Sus scrofa; Porcine (Pig)
胰腺再生蛋白Ⅳ(REG4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 4 (REG4)48T/96TY10443Mus musculus (Mouse)
蛋白Z依賴性蛋白酶抑制因子(ZPI)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Z Dependent Protease Inhibitor (ZPI)48T/96TY10444Rattus norvegicus (Rat)
白介素21(IL21)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 21 (IL21)48T/96TY10445Mus musculus (Mouse)
補體因子B(CFB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Complement Factor B (CFB)48T/96TY10446Homo sapiens (Human)
二氫嘧啶脫氫酶(DPYD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dihydropyrimidine Dehydrogenase (DPYD)48T/96TY10447Homo sapiens (Human)
D組著色性干皮病偶聯因子(XPD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Xeroderma Pigmentosum, Complementation Group D (XPD)48T/96TY10448Homo sapiens (Human)
趨化因子C-C-基元受體6(CCR6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chemokine C-C-Motif Receptor 6 (CCR6)48T/96TY10449Homo sapiens (Human)
高分子量激肽原(HMWK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for High Molecular Weight Kininogen (HMWK)48T/96TY10450Mus musculus (Mouse)
轉鐵蛋白(TRF)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transferrin (TRF)48T/96TY10451Rattus norvegicus (Rat)
亮氨酸豐富α2-糖蛋白1(LRG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)48T/96TY10452Rattus norvegicus (Rat)
α2-HS糖蛋白(αHSG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY10453Homo sapiens (Human)
抑制素B(INHB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Inhibin B (INHB)48T/96TY10454Rattus norvegicus (Rat)
胰島素樣生長因子結合蛋白1(IGFBP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1)48T/96TY10455Chicken (Gallus)
Ⅰ型膠原α2(COL1α2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Collagen Type I Alpha 2 (COL1a2)48T/96TY10456Canis familiaris; Canine (Dog)
Ⅰ型膠原α2(COL1α2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Collagen Type I Alpha 2 (COL1a2)48T/96TY10457Chicken (Gallus)
谷胱甘肽S轉移酶α1(GSTα1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Alpha 1 (GSTa1)48T/96TY10458Mus musculus (Mouse)
纖維蛋白原(FG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibrinogen (FG)48T/96TY10459Homo sapiens (Human)
補體成分2(C2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Complement Component 2 (C2)48T/96TY10460Homo sapiens (Human)
17-羥孕酮(17-OHP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for 17-Hydroxyprogesterone (17-OHP)48T/96TY10461General
雌三醇(E3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Estriol (E3)48T/96TY10462General
雄烯二酮(ASD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Androstenedione (ASD)48T/96TY10463General
胱天蛋白酶14(CASP14)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Caspase 14 (CASP14)48T/96TY10464Homo sapiens (Human)
胱天蛋白酶14(CASP14)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Caspase 14 (CASP14)48T/96TY10465Rattus norvegicus (Rat)
胱天蛋白酶14(CASP14)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Caspase 14 (CASP14)48T/96TY10466Mus musculus (Mouse)
表睪酮(ET)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Epitestosterone (ET)48T/96TY10467General
雌二醇(E2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Estradiol (E2)48T/96TY10468General
1,25-二羥基維生素D3(DHVD3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for 1,25-Dihydroxyvitamin D3 (DHVD3)48T/96TY10469General
衰變加速因子(DAF)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Decay Accelerating Factor (DAF)48T/96TY10470Homo sapiens (Human)
骨鈣素(OC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Osteocalcin (OC)48T/96TY10471Homo sapiens (Human)
骨鈣素(OC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Osteocalcin (OC)48T/96TY10472Rattus norvegicus (Rat)
骨鈣素(OC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Osteocalcin (OC)48T/96TY10473Danio rerio (Zebrafish)
降鈣素(CT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Calcitonin (CT)48T/96TY10474Homo sapiens (Human)
降鈣素(CT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Calcitonin (CT)48T/96TY10475Rattus norvegicus (Rat)
降鈣素(CT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Calcitonin (CT)48T/96TY10476Mus musculus (Mouse)
C組著色性干皮病偶聯因子(XPC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Xeroderma Pigmentosum, Complementation Group C (XPC)48T/96TY10477Homo sapiens (Human)
甲狀旁腺素受體2(PTHR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Parathyroid Hormone Receptor 2 (PTHR2)48T/96TY10478Homo sapiens (Human)
維生素D受體(VDR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vitamin D Receptor (VDR)48T/96TY10479Homo sapiens (Human)
心肌肌鈣蛋白I(TNNI3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Troponin I Type 3, Cardiac (TNNI3)48T/96TY10480Homo sapiens (Human)
肌酸激酶MB同工酶(CKMB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)48T/96TY10481Homo sapiens (Human)
肌紅蛋白(MYO)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Myoglobin (MYO)48T/96TY10482Homo sapiens (Human)
內皮素1(EDN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Endothelin 1 (EDN1)48T/96TY10483Rhesus monkey (Simian)
內皮素1(EDN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Endothelin 1 (EDN1)48T/96TY10484Mus musculus (Mouse)
各種類型ELISA測定時,一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。